human pancreatic carcinoma cell lines panc1  (ATCC)

Journal: bioRxiv

Article Title: BARTsc identifies key transcriptional regulators from single-cell omics data

doi: 10.64898/2026.02.24.707729

Figure Lengend Snippet: ( a ) UMAP visualization of the scMultiome dataset for human PDAC, where each point represents a single cell colored by cell type. ( b ) Signature analysis results for selected TRs in human PDAC. The heat map is colored by BARTsc score. Asterisks indicate significance level, *: p < 0.05, **: p < 0.01, ***: p < 0.001. ( c , d ) Performance comparison between BARTsc and other methods in identifying key regulators for each cell type from PDAC scMultiome dataset. Bar plots show the F1 scores for key regulator prediction for different methods across different cell types ( c ). Scatter plot shows the sensitivity and specificity of each method in identifying cell type key regulators ( d ). ( e - h ) UMAP visualizations of the ductal cells from the PDAC scMultiome dataset, where each point represents a single ductal cell colored by sub-clusters ( e ), or the normalized expression level of gene MKI67 ( f ), TOP2A ( g ) and CDK1 ( h ). ( i ) Scatter plot showing TRs ranked by BARTsc score. The top five predicted key regulators are highlighted and labeled. ( j ) Chronos scores quantifying the effects of CRISPR-mediated knockout of the top five predicted key regulators in PDAC cell lines. Each point represents a PDAC cell line, with lower Chronos scores indicating greater loss of cell fitness upon gene knockout. P-values are calculated by one-sided Wilcoxon rank-sum tests. ( k ) RT-qPCR results showing the relative mRNA levels of NELFA and selected genes upon NELFA knock-down by siRNA in PANC-1 cells. Bars represent the mean mRNA levels relative to those under control siRNA transfection groups after normalization to GAPDH mRNA levels, and error bars indicate the standard deviation. Asterisks indicate significance levels between siNELFA and siControl determined by one-sided t -test, *: p < 0.05, **: p < 0.01, ***: p < 0.001. ( l ) Growth of PANC-1 cells three days after NELFA knockdown (siNELFA) compared with the control cells (siControl). Error bars indicate the standard deviation. P-value is calculated by one-sided t -test.

Article Snippet: Human pancreatic carcinoma cell line PANC-1 was originally purchased from the American Type Culture Collection (ATCC) (Cat# CRL-1469).

Techniques: Single Cell, Comparison, Expressing, Labeling, CRISPR, Knock-Out, Gene Knockout, Quantitative RT-PCR, Knockdown, Control, Transfection, Standard Deviation

Journal: ACS Omega

Article Title: 5′-NucA-SMCC-DM1 and 5′-NucA-SPDMV-DM1 are Potent Aptamer–Drug Conjugates against Pancreatic Cancer

doi: 10.1021/acsomega.5c11364

Figure Lengend Snippet: Antiproliferative activity of synthesized NucA-DM1 conjugates in PANC-1, MIA PaCa-2, and MIHA. The cell viability curves of NucA-conjugates against (A) PANC-1 cells, (B) MIA PaCa-2 cells, and (C) MIHA cells were revealed by the CCK-8 assay as indicated within 72 h of incubation at 37 °C. Data were presented as mean ± SD of three independent experiments, and each was measured in triplicate ( n = 3). S.D., standard deviation.

Article Snippet: Human pancreatic epithelioid carcinoma cell line PANC-1 (RRID: CVCL_0480), human pancreatic cancer cell line MIA PaCa-2 (RRID: CVCL_0428), and immortalized human hepatocytes MIHA (RRID: CVCL_SA11) were obtained from the American Type Culture Collection (ATCC, USA) and cultured in Dulbecco’s Modified Eagle Medium (DMEM; Gibco), supplemented with 10% fetal bovine serum (FBS, Gibco) and 100 μg/mL penicillin and streptomycin (Gibco) at 37 °C in a humidified atmosphere comprising 5% CO 2 .

Techniques: Activity Assay, Synthesized, CCK-8 Assay, Incubation, Standard Deviation

Journal: ACS Omega

Article Title: 5′-NucA-SMCC-DM1 and 5′-NucA-SPDMV-DM1 are Potent Aptamer–Drug Conjugates against Pancreatic Cancer

doi: 10.1021/acsomega.5c11364

Figure Lengend Snippet: Cell cycle analysis was conducted through propidium iodide staining. The flow cytometry for the PANC-1 cells treated with (A) blank control, (B) DM1, and (C) 5′-NucA-SMCC-DM1, and the MIA PaCa-2 cells treated with (D) blank control, (E) DM1, and (F) 5′-NucA-SMCC-DM1 of different cell cycle phases, showed percentages of cell cycle phases on the right. Data were presented as mean ± SD of three independent experiments, and each was measured in triplicate ( n = 3). One-way ANOVA was used for statistical analysis, and the significance levels were indicated as * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001. ns, no significance. S.D., standard deviation.

Article Snippet: Human pancreatic epithelioid carcinoma cell line PANC-1 (RRID: CVCL_0480), human pancreatic cancer cell line MIA PaCa-2 (RRID: CVCL_0428), and immortalized human hepatocytes MIHA (RRID: CVCL_SA11) were obtained from the American Type Culture Collection (ATCC, USA) and cultured in Dulbecco’s Modified Eagle Medium (DMEM; Gibco), supplemented with 10% fetal bovine serum (FBS, Gibco) and 100 μg/mL penicillin and streptomycin (Gibco) at 37 °C in a humidified atmosphere comprising 5% CO 2 .

Techniques: Cell Cycle Assay, Staining, Flow Cytometry, Control, Standard Deviation

Journal: ACS Omega

Article Title: 5′-NucA-SMCC-DM1 and 5′-NucA-SPDMV-DM1 are Potent Aptamer–Drug Conjugates against Pancreatic Cancer

doi: 10.1021/acsomega.5c11364

Figure Lengend Snippet: Effect of conjugated DM1 on cellular binding and internalization by confocal microscopy. 250 nM 3′-Cy5-5′-NucA-SMCC-DM1 (red) was incubated with PANC-1, MIA PaCa-2, and MIHA cells, respectively, at 37 °C for 2 h. The nuclei were counterstained with Hoechst 33,342 (blue). Scale bar, 25 μm (the upper right black bar is the original bar, and the white bar is for better visualization). Error bars indicate mean ± SD ( n = 5 per group). Each replicate is from one biological experiment, quantified with 10 independent fields of view. One-way ANOVA was used for statistical analysis, and the significance levels were indicated as * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001. ns, no significance. S.D., standard deviation.

Article Snippet: Human pancreatic epithelioid carcinoma cell line PANC-1 (RRID: CVCL_0480), human pancreatic cancer cell line MIA PaCa-2 (RRID: CVCL_0428), and immortalized human hepatocytes MIHA (RRID: CVCL_SA11) were obtained from the American Type Culture Collection (ATCC, USA) and cultured in Dulbecco’s Modified Eagle Medium (DMEM; Gibco), supplemented with 10% fetal bovine serum (FBS, Gibco) and 100 μg/mL penicillin and streptomycin (Gibco) at 37 °C in a humidified atmosphere comprising 5% CO 2 .

Techniques: Binding Assay, Confocal Microscopy, Incubation, Standard Deviation

Journal: ACS Omega

Article Title: 5′-NucA-SMCC-DM1 and 5′-NucA-SPDMV-DM1 are Potent Aptamer–Drug Conjugates against Pancreatic Cancer

doi: 10.1021/acsomega.5c11364

Figure Lengend Snippet: Effect of conjugated DM1 on cellular binding and internalization. 250 nM 3′-Cy5-5′-NucA-SMCC-DM1 was incubated with three Alexa Fluor 488-labeled endocytic markers (transferrin, cholera toxin, and dextran; green) in PANC-1 cells, and the nuclei were counterstained with Hoechst 33,342 (blue). Scale bar, 10 μm (the lower right black bar is the original bar; the white bar is for better visualization). Error bars indicate mean ± SD ( n = 5 per group). Each replicate is from one biological experiment, quantified with 10 independent fields of view. One-way ANOVA was used for statistical analysis, and the significance levels were indicated as * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001. ns, no significance. S.D., standard deviation.

Article Snippet: Human pancreatic epithelioid carcinoma cell line PANC-1 (RRID: CVCL_0480), human pancreatic cancer cell line MIA PaCa-2 (RRID: CVCL_0428), and immortalized human hepatocytes MIHA (RRID: CVCL_SA11) were obtained from the American Type Culture Collection (ATCC, USA) and cultured in Dulbecco’s Modified Eagle Medium (DMEM; Gibco), supplemented with 10% fetal bovine serum (FBS, Gibco) and 100 μg/mL penicillin and streptomycin (Gibco) at 37 °C in a humidified atmosphere comprising 5% CO 2 .

Techniques: Binding Assay, Incubation, Labeling, Standard Deviation